Optical Rotatory Dispersion of Aldolase and Glyceraldehyde 3-Phosphate Dehydrogenase
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چکیده
منابع مشابه
Coenzyme-induced changes in the optical rotatory dispersion properties of glyceraldehyde 3-phosphate dehydrogenase.
The high binding affinity of diphosphopyridine nucleotide for glyceraldehyde S-phosphate dehydrogenase was first estimated quantitatively by Velick, Hayes, and Harting (1) by using an ultracentrifugation separation method. A dissociation constant of the order of 10-7 M (2) with a stoichiometry of 3 to 4 moles of DPN+ per mole of enzyme was determined (3). Several characteristics of the apoenzym...
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The catalytic interaction of glyceraldehyde-3-phosphate dehydrogenase with glyceraldehyde 3-phosphate has been examined by transient-state kinetic methods. The results confirm previous reports that the apparent Km for oxidative phosphorylation of glyceraldehyde 3-phosphate decreases at least 50-fold when the substrate is generated in a coupled reaction system through the action of aldolase on f...
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e.g. phosphoglucomutase, glucose 6-phosphate dehydrogenase and fructose 6-phosphate kinase. However, if the reaction occurs distant from the anomeric carbon atom of the substrate the enzymes show no anomeric specificity, e.g. hexokinase, glucokinase and glucose 6-phosphatase (Wurster & Hess, 1974~). A search for enzymes catalysing anomerization reactions leads to the following conclusion. For D...
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Fructose diphosphate aldolase and glyceraldehyde 3-phosphate dehydrogenase have been crystallized from rabbit livers after single or sequential injections of [‘“Cland (3H]lysine. The specific radioactivity of the lysine in the dehydrogenase rises continuously and reaches a maximum during the 5th day after injection, whereas that of the aldolase declines exponentially from the middle of the 1st ...
متن کاملThe active chemical state of D-glyceraldehyde 3-phosphate in its reactions with D-glyceraldehyde 3-phosphate dehydrogenase, aldolase and triose phosphate isomerase.
Glyceraldehyde 3-phosphate exists as the geminal diol and the free aldehyde in the molar ratio 29:1 in aqueous solution. The rate constant of the conversion of diol into aldehyde is 8.7x10(-2)sec.(-1) in the pH range 7.3-8.6 at 20 degrees . The free aldehyde is the substrate for d-glyceraldehyde 3-phosphate dehydrogenase. Over a wide concentration range of enzyme the rate of conversion of diol ...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1967
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)95993-4